PERIO - Periodontal Practice Today 5 (2008), No. 4 4. June 2009
PERIO - Periodontal Practice Today 4 (2007), No. 1 (09.03.2007)
TLR4-polymorphisms and the risk of chronic periodontitis
Reismann, Peter / Lichy, Christoph / Rudofsky, Gottfried / Nawroth, Peter P / Staehle, Hans-Jörg / Bierhaus, Angelika / Dörfer, Christof E
Objective: Polymorphisms of the toll-like receptor 4 (TLR4) have been reported to confer differences in the inflammatory response to gram-negative infections. Since periodontal diseases are caused by gram-negative microorganisms, we performed a pilot study to investigate the association of two polymorphisms (Asp299Gly and Thr399Ile) of the TLR4 gene with the risk of chronic periodontitis.
Study design: In a monocentric cross-sectional study, 75 caucasians with chronic periodontitis and 54 periodontally healthy controls were investigated. Asp299Gly and Thr399Ile genotypes were detected by polymerase chain reaction (PCR) and subsequent cleavage by NcoI and HinfI restriction endonucleases.
Results: In total, 9.3% (12/129) of the individuals, 13.3% (10/75) of the periodontitis group, and 3.7% (2/54) of the periodontally healthy controls were heterozygous for both polymorphisms. However, an increased prevalence of the Asp299Gly and Thr399Ile genotypes in the periodontitis group was shown, but it failed to reach the level of statistical significance either in the univariate method (p = 0.073) and also in the multivariate logistic regression model [odds ratio (OR): 4.000; 95%-confidence interval (95%CI) 0.839-19.061; p = 0.063].
Conclusions: Our results suggest that the presence of Asp299Gly and Thr399Ile polymorphisms of the TLR4 gene might have implications for the risk for chronic periodontitis, but larger studies are needed to confirm these data.
Keywords: bacterial infections, chronic periodontitis, gene regulation, inflammation, lipopolysaccharide receptor, TLR4