PERIO - Periodontal Practice Today 5 (2008), No. 4 4. June 2009
This six-month longitudinal study quantified gingival crevice fluid elastase and interleukin-8 and analysed their relationship to clinical outcome of mechanical therapy. Eighteen patients with chronic periodontitis, presenting 209 sites, were included in the study (123 periodontitis, 45 gingivitis and 41 healthy sites). After oral hygiene instructions and supragingival scaling, periodontitis sites were root planed. Gingival crevice fluid was collected twice before treatment within 10 days, and two weeks, two and six months thereafter. Elastase was determined using a fluorogenic substrate, and interleukin-8 was quantified using enzyme-linked immunosorbent assay. Clinical parameters included pocket probing depths, bleeding on probing and plaque scores. Therapy resulted in highly significant reductions of elastase and interleukin-8 in all three categories of sites. Eleven periodontitis sites, distributed in six subjects, remained at six months with pockets greater than 5mm. At baseline and at two months these sites showed significantly higher levels of pocket probing depths and bleeding on probing (p < 0.01), whereas no difference was noted for elastase and interleukin-8. On the subject level, however, no difference was noted at baseline between these six patients and the other ones. Clinical improvement after periodontal therapy was associated with a highly significant reduction in elastase and interleukin-8 levels. Whereas probing depth and bleeding on probing at baseline reflected the clinical outcome, the biochemical parameters did not.
Keywords: scaling and root planing, predictive value, elastase, interleukin-8, gingival crevice fluid